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RESEARCH ASSISTANT

University of Iowa Hospitals and Clinics
Iowa City

Job Description

BASIC FUNCTION AND RESPONSIBILITY

This person will work in the Biochemistry research laboratory of David Price. Exciting projects in the lab use a combination of biochemical, molecular, and cellular approaches that address the regulation of human and viral transcription, especially RNA polymerase II elongation control. This person will be responsible for the generation and interpretation of experimental data and assist in the daily operations of the laboratory.

CHARACTERISTIC DUTIES AND RESPONSIBILITIES

Propagation of human cell lines

Protein purification, SDS-PAGE, and Western blot analysis

DNA plasmid construction and mutagenesis (PCR, DNA purification, bacterial transformations)

Perform library research on topics studied, prepare research materials for reports, and assist in the preparation of papers for publication.

Order general laboratory supplies, maintain equipment, and sustain laboratory organization.

Analyze and interpret data with the help of others in the laboratory, and determine the validity of the results. Keep detailed laboratory notebooks recording experimental data and analyses and present these findings at weekly lab meetings. Recommend further experiments and/or changes in procedures.

Other duties as assigned.

Qualifications

Preparation of buffer solutions and common molecular biological reagents in the laboratory

Gel electrophoresis of DNA, RNA, and proteins

Cell culture

Demonstrated excellent written and verbal communication skills

5. Proficiency in computer use (Windows and Office applications)

6. Knowledge of practices, behavior, applicable laws, rules and regulations governing ethical research conduct.

Salary

$37,313.00 to Commensurate

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